Undaria pinnatifida (Wakame) Intake Ameliorates High-Fat Diet-Induced Glucose Intolerance via Promoting GLUT4 Expression and Membrane Translocation in Muscle

Figure 2

Effect of wakame components on AKT and AMPK phosphorylation and GLUT4 levels in C2C12 cells. Differentiated C2C12 cells were treated with PBS, DMSO, metformin (1 mM), fucoidan (1 mg/mL), ethanol extract (50 μg/mL), hexane extract (50 μg/mL), fucoxanthinol (1 μg/mL), or wakame peptide (100 μg/mL) for 24 h before lysis. The lysates were analyzed with Western blot using (a) GLUT4, (b) P-AKT (s473) and T-AKT, and (c) P-AMPK (T172) and T-AMPK antibodies and quantified. The level of GLUT4 was normalized by that of GAPDH. The intensity of P-AKT and P-AMPK bands was normalized by that of their respective total protein bands. The data are presented as mean ± SD (n = 4). The data were analyzed with one-way ANOVA combined with the Tukey test. , compared to PBS; #, compared to DMSO.

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