Undaria pinnatifida (Wakame) Intake Ameliorates High-Fat Diet-Induced Glucose Intolerance via Promoting GLUT4 Expression and Membrane Translocation in Muscle

Figure 6

Wakame’s effect on level and phosphorylation of insulin signaling molecules and GLUT4 in skeletal muscle. The skeletal muscle cells were collected via dissection from the ND, ND + W, HFD, and HFD + W mice. The cell lysates were analyzed by Western blotting with (a) GLUT4, (b) P-AKT (s473) and T-AKT, and (c) P-AMPK (T172) and T-AMPK antibodies. The level of GLUT4 was normalized to that of GAPDH. The level of P-AKT and T-AMPK was normalized to that of their respective total protein. The data were presented as mean ± SD (n = 4-5) and analyzed with one-way ANOVA combined with the Tukey test. .

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