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Journal of Nanotechnology
Volume 2012 (2012), Article ID 196189, 7 pages
http://dx.doi.org/10.1155/2012/196189
Research Article

Uptake of Single-Walled Carbon Nanotubes Conjugated with DNA by Microvascular Endothelial Cells

1Department of Biomedical Sciences, Missouri State University, Springfield, MO 65897, USA
2Department of Physics, Astronomy, and Materials Science, Missouri State University, Springfield, MO 65897, USA
3Department of Biology, Missouri State University, Springfield, MO 65897, USA

Received 26 July 2011; Accepted 13 August 2011

Academic Editor: Dongwoo Khang

Copyright © 2012 Joseph Harvey et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Single-walled carbon nanotubes (SWCNTs) have been proposed to have great therapeutic potential. SWCNTs conjugated with drugs or genes travel in the systemic circulation to reach target cells or tissues following extravasation from microvessels although the interaction between SWCNT conjugates and the microvascular endothelial cells (ECs) remains unknown. We hypothesized that SWCNT-DNA conjugates would be taken up by microvascular ECs and that this process would be facilitated by SWCNTs compared to facilitation by DNA alone. ECs were treated with various concentrations of SWCNT-DNA-FITC conjugates, and the uptake and intracellular distribution of these conjugates were determined by a confocal microscope imaging system followed by quantitative analysis of fluorescence intensity. The uptake of SWCNT-DNA-FITC conjugates (2 μg/mL) by microvascular ECs was significantly greater than that of DNA-FITC (2 μg/mL), observed at 6 hrs after treatment. For the intracellular distribution, SWCNT-DNA-FITC conjugates were detected in the nucleus of ECs, while DNA-FITC was restricted to the cytoplasm. The fluorescence intensity and distribution of SWCNTs were concentration and time independent. The findings demonstrate that SWCNTs facilitate DNA delivery into microvascular ECs, thus suggesting that SWCNTs serving as drug and gene vehicles have therapeutic potential.