Figure 2: Differentiation of MSCs cultured on nanofibers in OIM or AIM for 14 days. (A) Phase contrast images of differentiated MSCs. ((a)–(c)) MSCs differentiated with OIM, stained by von Kossa method and ((d)–(f)) MSC differentiated with AIM, stained by Oil-Red-O. Cells were cultured on a flat dish ((a) and (d)), aligned fibers ((b) and (e)), and random fibers. ((c) and (f)) Bar = 500 μm. (B) Quantitative analysis of osteoinduction. (C) Quantitative analysis of adipoinduction. (*: 𝑃 < 0 . 0 5 ).