Targeting Antibodies to Carbon Nanotube Field Effect Transistors by Pyrene Hydrazide Modification of Heavy Chain Carbohydrates
Figure 1
Reactions performed to modify antibody carbohydrates with pyrene hydrazide, illustrated with galactose. Step 1: antibodies are treated with 10 mM NaIO4, which oxidizes cis-diols to aldehyde groups. Step 2: after a desalting step, the antibody, it is reacted with 1-pyrenebutanoic acid hydrazide which forms stable hydrazone bonds with the formed aldehyde groups.
