Journal of Nanotechnology / 2012 / Article / Tab 2

Research Article

Manufacturing Strategy for Multiwalled Carbon Nanotubes as a Biocompatible and Innovative Material

Table 2

Fractionation rate using two fluorescent reagents for tROS/RNS with FCM on BEAS-2B cells exposed to MWCNTs at 1 and 24 h.
(a) Fractionation rate (%) of 10,000 cells at 1 h (mean ± SE, 𝑛 = 3 )

FractionControlNT15NT15+FeNT15-30NT15-26NT15-22NT15-13

tROS 3 . 8 ± 0 . 4 5 . 3 ± 0 . 7 5 . 9 ± 0 . 4 5 . 1 ± 0 . 6 5 . 1 ± 0 . 2 4 . 5 ± 0 . 3 1 0 . 0 ± 0 . 2
Superoxide 9 . 9 ± 0 . 2 5 . 7 ± 0 . 1 5 . 0 ± 0 . 6 3 . 7 ± 0 . 2 5 . 5 ± 0 . 5 5 . 5 ± 0 . 7 1 3 . 2 ± 2 . 2
Peroxide 2 . 0 ± 0 . 2 5 . 7 ± 1 . 2 8 . 4 ± 1 . 3 7 . 6 ± 1 . 4 5 . 9 ± 0 . 7 4 . 6 ± 0 . 9 8 . 6 ± 1 . 3
Negative 8 4 . 3 ± 0 . 3 8 3 . 3 ± 1 . 8 8 0 . 7 ± 1 . 2 8 3 . 6 ± 2 . 0 8 3 . 5 ± 0 . 3 8 5 . 4 ± 0 . 7 6 8 . 2 ± 1 . 0

FractionPyocyaninNT08NT08+FeNT08-30NT08-26NT08-22NT08-13

tROS 4 2 . 2 ± 2 . 1 6 . 6 ± 0 . 8 7 . 6 ± 0 . 6 7 . 3 ± 0 . 6 8 . 1 ± 0 . 3 7 . 7 ± 0 . 8 9 . 2 ± 0 . 4
Superoxide 1 . 8 ± 0 . 3 3 . 7 ± 0 . 5 2 . 8 ± 0 . 3 3 . 9 ± 1 . 0 4 . 0 ± 0 . 7 5 . 0 ± 0 . 8 6 . 3 ± 0 . 7
Peroxide 2 6 . 7 ± 1 . 5 1 0 . 7 ± 0 . 8 1 5 . 1 ± 1 . 6 1 2 . 4 ± 2 . 5 1 1 . 3 ± 1 . 6 1 0 . 5 ± 2 . 0 1 1 . 9 ± 1 . 4
Negative 2 9 . 3 ± 2 . 6 7 9 . 0 ± 1 . 3 7 4 . 5 ± 1 . 9 7 6 . 4 ± 1 . 9 7 6 . 6 ± 1 . 0 7 6 . 8 ± 2 . 0 7 2 . 6 ± 0 . 9

(b) Statistical significance compared between different types of MWCNTs by the Tukey-Kramer method (mean ± SE, 𝑛 = 3 , * 𝑃 < 0 . 0 5 , ** 𝑃 < 0 . 0 1 )

FractionMWCNT-150MWCNT-80

tROSControl versus NT15+Fe*, NT15-13** Control versus NT08+Fe*, NT08-30*, NT08-26**, NT08-22*, NT08-13**
NT15-13 versus NT15**, NT15+Fe**, NT15-30**, NT15-26**, NT15-22**

SuperoxideConrol versus NT15**, NT15+Fe*, NT15-30**, NT15-26**, NT15-22*Control versus NT08**, NT08+Fe**, NT08-30*, NT08-26*, NT08-22*, NT08-13*,
NT15-13 versus NT15**, NT15+Fe**, NT15-30**, NT15-26**, NT15-22**NT08+Fe versus NT08-13*

PeroxideControl versus NT15+Fe*, NT15-26*, NT15-13*Conrol versus NT08**, NT08+Fe*, NT08-26*, NT08-13*

NegativeControl versus NT15-13*Conrol versus NT08*, NT08+Fe*, NT08-26*, NT08-13**
NT15-13 versus NT15**, NT15+Fe**, NT15-30**, NT15-26**, NT15-22**

(c) Fractionation rate (%) of 3,000 or fewer cells at 24 h (mean ± SE, 𝑛 = 3 )

FractionControlNT15#NT15+Fe#NT15-30#NT15-26NT15-22NT15-13#

tROS 3 . 4 ± 2 . 1 6 . 3 ± 0 . 6 7 . 1 ± 0 . 6 1 1 . 1 ± 1 . 8 7 . 0 ± 0 . 4 7 . 4 ± 0 . 9 1 8 . 9 ± 3 . 4
Superoxide 5 . 9 ± 0 . 3 1 7 . 9 ± 2 . 6 1 4 . 5 ± 1 . 0 1 9 . 8 ± 2 . 0 1 7 . 7 ± 3 . 2 1 7 . 6 ± 2 . 6 2 0 . 1 ± 1 . 7
Peroxide 2 . 0 ± 1 . 5 4 . 7 ± 0 . 2 7 . 3 ± 0 . 6 8 . 9 ± 0 . 9 5 . 8 ± 0 . 2 5 . 5 ± 1 . 0 8 . 9 ± 1 . 8
Negative 8 8 . 7 ± 3 . 5 7 1 . 1 ± 2 . 1 7 1 . 2 ± 1 . 6 6 0 . 2 ± 4 . 2 6 9 . 5 ± 3 . 1 6 9 . 5 ± 1 . 9 5 2 . 1 ± 3 . 8

FractionPyocyaninNT08NT08+FeNT08-30NT08-26NT08-22NT08-13

tROS 5 0 . 5 ± 2 . 3 9 . 9 ± 1 . 0 9 . 9 ± 2 . 3 9 . 6 ± 2 . 2 1 0 . 1 ± 1 . 8 9 . 9 ± 1 . 3 1 0 . 7 ± 1 . 5
Superoxide 2 0 . 8 ± 0 . 7 2 1 . 5 ± 1 . 9 1 7 . 6 ± 1 . 4 1 7 . 3 ± 1 . 6 2 5 . 3 ± 1 . 3 2 8 . 7 ± 2 . 5 4 1 . 7 ± 3 . 1
Peroxide 1 . 3 ± 0 . 1 4 . 2 ± 0 . 8 5 . 6 ± 1 . 3 4 . 3 ± 1 . 6 3 . 0 ± 0 . 6 2 . 5 ± 0 . 4 1 . 4 ± 0 . 4
Negative 2 7 . 4 ± 1 . 7 6 4 . 5 ± 0 . 3 6 6 . 9 ± 2 . 3 6 8 . 8 ± 2 . 2 6 1 . 6 ± 1 . 4 5 8 . 9 ± 0 . 9 4 6 . 2 ± 1 . 4

# = less than 3,000 cells.

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