Silica Sol-Gel Entrapment of the Enzyme Chloroperoxidase

Figure 5

Absorbance spectra of sol-gel entrapped CPO (solid line) and free CPO (dashed line). The silica sol-gel sheet was ~1 mm thick and contained 1.6 mg/mL CPO. The sheet was placed on the wall of a 10 mm wide plastic cuvette and immersed in 50 mM potassium phosphate buffer, pH 6.0. The solution reference sample was prepared from the same CPO stock via dilution to 0.16 mg/mL (4 μM) with 50 mM potassium phosphate buffer, pH 6.0 in a 10 mm thick plastic cuvette.