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Journal of Nanotechnology
Volume 2017, Article ID 8490730, 7 pages
https://doi.org/10.1155/2017/8490730
Research Article

Silicone Doped Chitosan-Acrylamide Coencapsulated Urea Fertilizer: An Approach to Controlled Release Fertilizers

Department of Distance Education, Institute of Adult Education, P.O. Box 20679, Dar es Salaam, Tanzania

Correspondence should be addressed to Sempeho Ibahati Siafu; zt.ca.eai@ohepmes

Received 2 September 2016; Revised 19 January 2017; Accepted 23 January 2017; Published 12 February 2017

Academic Editor: María J. Lázaro

Copyright © 2017 Sempeho Ibahati Siafu. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

In the absence of special management practices, urea is known to undergo chemical transformations resulting in severe losses (≈60–70%) of total fertilizer applied. In an attempt to design urea controlled release fertilizers in order to counterbalance the 60–70% loss, urea was cross-linked with chitosan and acrylamide under refluxed in situ copolymerization technique; the procedures were repeated with silicone doping prior cross-linking with MBA. The particles were characterized with FTIR/ATR, EDX, XRD, and SEM. The IR bands observed within 3426–409 cm−1 revealed the formation of new bands after coencapsulation for the N-H, N-H, OH, NH2, CH2, C=O, ′NH2, C=C, NH2, C-N, CH3, $C-N, NH2, C=O, and $CH2. Crystallinity indices for urea with and without silicone doping were found to be 50.9% and 72.1%, respectively, with a distinctive split peak at () 12.30°. The formation of Microdunes and Microballs 3D network sized 0.64 μm was noted. Release profiles demonstrated that 80% N was released in a period of 30 days at RT and pH 7. The release patterns exhibited linear and deformed sigmoid release models. Empirically, the findings demonstrated that it is possible to design urea controlled release fertilizers with varying particle sizes and morphologies by using chitosan-acrylamide coencapsulation.

1. Introduction

Owing to its being a low cost nitrogen fertilizer with a high nitrogen content of about 46%, urea fertilizer has been used (within Tanzania in particular) to supplement crop plants of nitrogen [13]. Urea has been useful in horticultural practices, enrichment of lawns or sods, meadows, prairies, and road-side protective grasses necessary to control erosion by the road sides, orchards, in cereals plantation, and so forth, such that its advanced research is vital for the economic growth of any country like Tanzania whose economy depends on agriculture [48].

On the contrary, despite being such an economically and agronomically useful chemical material urea is limited for the reason that: its availability to crop plants is less than 50% with up to 60–70% loss of the sum applied in the soil due to vaporization, hydrolysis, and quick leaching rendering its fertilizer use efficiency (FUE) low [3, 9]. As a consequence, attempts involving the use of excipients molecules to obtain controlled release urea formulations have been done to maximize the efficiency and availability to crop plants to about 70% or more. These include the synthesis of urea-aldehyde condensation products, such as urea-formaldehyde (UF), urea triazone (UT), crotonylidene diurea (CDU), and chemically decomposing compounds, such as isobutylidenediurea (IBDU) [10] and urea granules coated by hydrophobic polymers or as matrices such as polyolefins and rubber and gel-forming polymers (hydrogels) which are hydrophilic in nature and sulphur-coated urea (SCU).

Essentially, several polymers and polymer resins are known to be used in the designing of urea controlled release fertilizer (CRF) formulations including acrylamide, urethane resin, epoxy resin, alkyd resin, unsaturated polyester resin, phenol resin, urea resin, melamine resin, phenol resin, and silicon resin along with a good number of natural biopolymeric materials such as chitosan which has been used in this study [10]. To the best our knowledge, no attempts have ever been done to design urea CRF formulations by using copolymerization of chitosan and acrylamide along with silicone doping. In that case, we have described in this paper the preparation of urea CRF formulation as an approach to controlled release systems (CRSs) using simple and inexpensive starting materials including silicone oil, chitosan biopolymer, acrylamide, and urea synthetic fertilizer.

Chitosan was used in this study owing to its inherent properties such as high swelling ratio [11], water-retention capacity [12], antimicrobial activity, availability, low toxicity, biodegradability, capability for high cross-linking, and low processing cost [1315]. Also, the nanoparticles resulting from chitosan are known to exhibit spherical shapes and uniform sizes [16]; similarly, the complexes formed between chitosan with other bioactive species (urea in our context) along with other polymers are known in modifying the release characteristics of the named CRSs formulated [17]. On the other hand, we have stated elsewhere [10] that “polyacrylamide is known to reduce soil erosion” and also it is biodegradable [18, 19]; thus polyacrylamide was chosen to be used along with chitosan in the urea copolymerization process.

In due course, the synthesized urea CRF formulation is expected to augment crops grain yield and straw yield as well as enrich soil with nitrogen thereby increasing its nutritional status and also the efficacy of Conventional Chemical Fertilizers (CCFs) such as urea [20]. In fact, the efficacy of CRFs is known to be more than four times higher than CCFs which indicates that even 1/4th of chemical fertilizers can be made more effective with organic blending into CRSs [20].

2. Materials and Methods

2.1. Materials

Urea (ACS reagent, 99.0–100.5%) was supplied by Sigma Aldrich, deionized distilled (DI) water was supplied by Professor Kim’s lab, chitosan powder was supplied by Sigma Aldrich, acetic acid glacial (99.7%) was supplied by Duksan Pure Chemicals, Ansan city, Kyungki-do, Korea, 2,2′-azobisisobutyronitrile (AIBN) (≥98.0%) was supplied by Samchun Pure Chemicals Co. Ltd., Gyonggi-do, Korea, CCl4 (≥99.0%) was supplied by Junsei Chemical Co., Ltd., Japan, N,N′-methylenebisacrylamide (MBA) (≥98.0%) was supplied by Samchun Pure Chemicals Co. Ltd., Gyonggi-do, Korea, acrylamide (≥98.5%) was supplied by Samchun Pure Chemicals Co. Ltd., Gyonggi-do, Korea, n-hexane was supplied by was supplied by Sigma Aldrich, and silicone oil was supplied by Sigma Aldrich.

2.2. Methods
2.2.1. Preparation of Chitosan Solution

A chitosan solution (2%) was prepared by dissolving 10 g chitosan powder into 500 mL of 10% glacial acetic acid to get the pH of the solution in a range of 4-5. The solution was stirred for 40 hours at RT until a gold colour was obtained.

2.2.2. Preparation of Chitosan-Acrylamide Encapsulated Urea

AIBN (0.2 g) was agitated with 30 mL of CCl4 in the 3-necked round bottomed flask (RBF) containing 0.4 g of MBA and the apparatus was set under hood and was run for 20 minutes under N2 atmosphere at 60°C. Thereafter, a chitosan solution prepared which contained 7 g of acrylamide and 8 g of urea was introduced and stirred under reflux overnight. The product was washed with n-hexane and dried at 60°C with a vacuum oven; it was then labelled as CPAM-1.

2.2.3. Preparation of Chitosan-Acrylamide Encapsulated Urea with Silicone Oil Doping

The procedures for the preparation of chitosan-acrylamide encapsulated urea (described above) were repeated with the following modifications. Stirring under reflux was done for six hours after which 1 mL of silicone oil was spiked with a syringe as dopant and then continued stirring overnight. The product was washed with n-hexane and dried under vacuum oven and it was then labelled as CPAM.

2.2.4. Release Experiments

Release experiments were carried out in water (pH 7) as media as follows. 5 grams of the prepared CRFs was dissolved in 100 cm3 of distilled water at room temperature (RT). The content of released urea from the encapsulated fertilizer was collected by using column extraction method in which a PVC-based column measuring 30 cm long and 5 cm in diameter was fitted with gauze at the bottom end and then capped. The procedures were adopted with modification from [21]. Each column was leached at an interval of 24 hours for a period of 30 days owing to the fact that a pilot experiment had revealed that about 80% of fertilizer could be released in that time range. The amount of nitrogen contained in the leachate was determined by using Kjeldahl method (total Kjeldahl N) which involves three steps, namely, digestion, distillation, and titration. Each experiment was done in triplicate for each sample and the average values were used for plotting the release profiles.

2.2.5. Characterization

Infrared Spectroscopy. FTIR-ATR analysis was performed at wavelengths between 7500 and 360 cm−1 using a Bruker Optic GmbH (alpha model, Laser class 1) Spectrometer with attenuated total reflectance.

X-Ray Diffraction. The XRD analysis was carried out using Rigaku Corp. D/Max-2500/PC X-ray Diffractometer was equipped with a back monochromator operating at 40 kV and 100 mA at the scanning range of 5°–80° with a step size of 0.1° and a time/step of 1 s using copper cathode (Cu Kα1) as the X-ray source (λ = 1.54056 Å).

Scanning Electron Microscopy (SEM). The samples surfaces morphology was investigated by using Field Emission Scanning Electron Microscopy (Hitachi S-4800, Japan). The accelerated voltage was 15 kV.

Particle Size Analysis. Image Tool software was used to analyze the size of particles from the SEM images. The plot for the average particles sizes was performed thereafter using Origin Pro 8 software. Kjeldahl apparatus was used for determining the amount of nitrogen released from CRFs.

3. Results and Discussion

3.1. Vibrational Spectra

The infrared spectra of urea as well as of the samples CPAM and CPAM-1 are shown in Figure 1 whereas that of chitosan is given away in Figure 2. A summary of band assignments for urea, CPAM, and CPAM-1 samples is given in Table 1. Detailed band assignments for chitosan can be found elsewhere [22]. Typically, silicones have strong characteristics absorption bands in the mid infrared spectra range at 3000–2900, 1500–1490, 1260–1250, 1100, 1080–1000, 770, 751-750, and 600 cm−1 [2325]. The characteristics absorption peaks for the acrylamide are known to exist around 3365, 3352, 3180, 3030, 3011, 1675, 1654–1650, 1612, 1451, 1430, 1353, 1325, 1282, 1138, 1053, 991, 963, 831, 816, 708, 660, 634–626, 490, and 225 cm−1 [2628], whereas those of chitosan biopolymer (Figure 2) occur around 3750, 3367, 3350, 2927–2900, 2875, 1680, 1662, 1605, 1559, 1480, 1426, 1393, 1375, 1333, 1320, 1164–1150, 1092–1080, 1042, 1025, and 896–890 cm−1 [22, 26, 2931]. For pure urea fertilizer, the absorption bands are clearly seen in Table 1 which is approximately around the values we reported in [9, 10].

Table 1: Band assignment for CPAM, urea, and CPAM-1.
Figure 1: FTIR/ATR absorption band for urea CPAM-1 and CPAM.
Figure 2: FTIR/ATR absorption band for chitosan biopolymer.

As indicated in Table 1, there were variations in the characteristic absorption bands observed relative to their original starting materials; this has revealed the fact that new bonds might have been formed as a result of copolymerization between chitosan-acrylamide with urea fertilizer and later with silicone oil used as dopant. The silicone doping was observed to be successful due to disappearance of typical silicone bands; however, the backbone of silicone structure was noticed due to the presence of its characteristics peaks around 1258, 1081, and 1013 cm−1. As seen in Figure 1, these characteristics bands were only seen in the CPAM; and they were absent in the CPAM-1 as a result of silicone doping.

The FTIR/ATR analysis also indicated encapsulation of urea fertilizer with copolymerization of chitosan and acrylamide materialized. Upon deducing peaks observed in Figure 1, it can easily be said that, among all characteristics of urea absorption bands, only bands at 3326 and 1458 cm−1 were unaltered after encapsulation. Besides, there were observable variations to these bands that the absorption band at 3326 cm−1 got transformed to 3339 cm−1 (for CPAM) and 3337 cm−1 (for CPAM-1) after encapsulation. This pointed out that the N-H in-phase bond stretching as well as the in-phase CH3 bending vibrations were present but the outer N-H bonds were completely covered with encapsulant such that the N-H out-phase bond stretching was absent after encapsulation. It was thus alleged that silicone caused a slight band shift from 3337 to 3339 cm−1 and from 1447 to 1448 cm−1 for CPAM-1 and CPAM, respectively.

3.2. X-Ray Analysis

The XRD patterns for the CPAM and CPAM-1 were studied in the 2θ range of 5° to 50°. The findings as shown in Figure 3 indicated that all the samples were more or less amorphous whereas the level of crystallinity was low. Based on the methods described by [47] crystallinity index (CI) was calculated from the ratio of the height of the 002 peak (I002) and the height of the minimum. That said, the CI was calculated for both CPAM and CPAM-1; the results indicated that CPAM-1 was more crystalline than CPAM; their respective crystallinity indices were 72.1% and 50.9%. The peaks observed for CPAM were at 12.30° (d) and 22.5° (s); both were broad and distinct. For CPAM-1, one high intensity peak was observed at 19.0° (d) and another broad peak at 22.5°. The pattern of these broad peaks illustrated that the peak for CPAM-1 had a wider shoulder as compared to CPAM.

Figure 3: The X-ray diffraction pattern for CPAM-1 and CPAM.

Essentially, the extra split peak (d) at 12.30° was considered to be distinctive for the CPAM and it can be used to identify CPAM from CPAM-1. The appearance of the split peak (d) at 12.30° for the CPAM was ascribed to the presence of OH and NH2 groups from the chitosan which form stronger intermolecular hydrogen bonds upon copolymerization. The presence of silicone dopant which covered these functional groups caused a disappearance of this peak such that it was completely absent in the CPAM-1. This suggested the fact that the backbone structure of chitosan was not deformed during copolymerization process but with silicone doping the backbone structure which disappeared indicating the existence of a strong interaction between chitosan-acrylamide-urea and silicone. Reflecting on the findings by [48], the waveband pattern observed for the CPAM curve revealed that chitosan structure acted as a copolymer framework during the coencapsulation process such that its original known structure [48] got modified into the waveband pattern observed in Figure 3. Similarly, in the CPAM the amorphous phase seemed to dominate the crystalline phase; this may suggest the impact of silicone on the crystallinity of the prepared composite CPAM-1.

3.3. SEM and Particle size Analysis

For the purpose of determining the particle size and sample morphology SEM images were taken and analyzed (Figures 46). The findings indicated that when urea fertilizer was cross-linked through copolymerization of acrylamide and chitosan biopolymer under the specified experimental conditions, the resulting composite materials exhibited what we have termed as the “Microballs” appearance. As can be seen in Figure 4, the Microballs were observed to be highly cross-linked and that each particle was strongly bonded to the other in a 3D network to form a beautiful sheet of numerous Microballs. Besides, when silicone was doped into the matrices of Microballs 3D network, a complete structural transformation was observed (Figure 5); a new phase (which was named “Microdunes”) was observed to emerge following silicone doping. Indeed, a very beautiful wrinkled sheet of Microdunes developed at the expense of the Microballs thereby indicating the impact of silicone doping on the framework of CPAM-1 composite materials.

Figure 4: The SEM micrograms for the CPAM-1 showing the Microballs architecture.
Figure 5: The SEM micrograms for the CPAM showing highly wrinkled surfaces: the Microdunes.
Figure 6: The average particle size of CRFs Microballs.

Comparatively, the surfaces of the Microballs were seen to be smooth while those of the Microdunes were seen to be rough or rather wrinkled. By instinct, this observation revealed that the surface area of the Microdunes could be higher than that of the Microballs. Besides, the Microballs were observed to be highly cross-linked and interconnected than the Microdunes. That is to say, for the purpose of designing CRF formulations with roundish particles along with maximum cross-linking, the CPAM-1 architecture could be more favourable whereas if a need is to achieve a maximum surface area, CPAM structural pattern could be more favourable. Owing to that, the choice of a synthetic method will depend on the desired CRF formulation as per the intended usage. Particle size analysis of the Microballs indicated that the average particles sizes as described in the mean region (Figure 6) were about μm.

3.4. Nitrogen Release

Release experiments were performed as described earlier using tap water as media without further treatment. Results pertaining to the percentage of N-released with time for a period of 30 days are presented in Figure 7.

Figure 7: Release profiles for urea, CPAM, and CPAM-1 as a function of time at RT (pH 7).

Essentially, urea release from the conventional urea fertilizer can be seen as a fast reaction which was completed in just the first day of the release trials. For CPAM-1, the release model was a linear type whereas for the CPAM the release model presented a kind of deformed sigmoid pattern due to appearance of some distorted steps in the curve’ shape. As explained elsewhere in these experimental series [10], the identification of the release pattern follows the shape, lag, and lock off of the curves established. Typically, nutrient release occurs in phases [10] but in short, the phases involve water penetration creating swelling of the excipients followed by the nutrient release by a way of diffusion or connective flows via pores developed in the surfaces of the excipients. That said, the shape and the size of the resultant CRFs contribute to the release patterns, either fast release, moderate, or delayed release. In this study, morphological differences between the Microballs and Microdunes could help explain the differences in release pattern observed in Figure 7. As can be seen, sigmoid release pattern can be linked with Microdunes (for CPAM) based structures whereas the roundish Microballs (for CPAM-1) relates to linear release pattern. This study has not been able to establish the reason behind these observations, thereby calling for further investigations. According to CEN’s standards, a fertilizer formulation can only be considered as a CRF if 75% of the nutrients contained can be released in 28 days at room temperature. That said, the release pattern observed in Figure 7 shows that the Microdunes and Microballs may be considered as controlled release fertilizer formulations [3].

4. Conclusion

Pragmatically, a clear discussion on the urea CRF formulations prepared by using chitosan, acrylamide, and urea copolymerization along with silicone doping was given. It was observed that the CRFs exhibit superior characteristics over the CCFs and so the amendments of urea CCFs via copolymerization of chitosan and acrylamide were a key step toward its commercialization as well as enhanced FUE. Significant morphological variations were observed between the properties of urea cross-linked with and without silicone doping, thereby indicating the possibility of designing the urea fertilizer forms as per intended usage based on the climatic differences and the type of field application. The study has left several unattempted questions including the question of thermokinetic properties of the prepared CRFs formulations, release profiles under actual soil conditions with varying pH, and properties as well as the questions regarding the formation of linear and deformed sigmoid release pattern following silicone doping. Nevertheless, the findings revealed that amendments of urea CCFs into the urea CRF formulations using excipients emanating from chitosan-acrylamide coencapsulation along with silicone doping are a feasible process.

Competing Interests

The author declares that there is no conflict of interests regarding the publication of this paper.

Acknowledgments

The author would like to thank Miss Niconia Amos Bura and the Institute of Adult Education, Tanzania, for their technical support in the preparation of this paper.

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