Research Article
Biological Effect of Organically Coated Grias neuberthii and Persea americana Silver Nanoparticles on HeLa and MCF-7 Cancer Cell Lines
Table 1
Oligonucleotide sequences for quantitative, real-time PCR analysis.
| | Gene name | Sequences (5′-3′) | Tm (°C) | GenBank (accession code) | Amplicon size (bp) | Efficiency | Reference |
| | Glutathione-S-transferase (GST) | Forward: GATACTGGGGTACTGGGACATCC | 61.58 | NM_146421.2 | 130 | 1.87 | [31] | | Reverse: CCACTGGCTTCTGTCATAATCAGG | 61.22 | | Probe: CCCACGCCATCCGCCTGCTCCT | 68.9 |
| | Tumor suppressor p53 | Forward: TAACAGTTCCTGCATGGGCGGC | 65.66 | NM_000546.5 | 121 | 1.96 | [32] | | Reverse: AGGACAGGCACAAACACGCACC | 66.01 | | Probe: CGGAGGCCCATCCTCACCATCATCA | 67.97 |
| | Actin beta (ACTB) | Forward: CCTCGCCTTTGCCGA | 56.04 | NM_001101.3 | 171 | 2.23 | [33] | | Reverse: TGGTGCCTGGGGCG | 58.15 | | Probe: CCGCCGCCCGTCCACACCCGCC | 69.7 |
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