Cell Cycle Regulating Kinase Cdk4 as a Potential Target for Tumor Cell Treatment and Tumor Imaging

Table 1

Primer sequences and annealing temperatures for quantitative real-time RT-PCR.

Primer	bp pos.	𝑇annealing/°C	Sequence
β-actin-f	736 – 755	59	5^′-GGA CTT CGA GCA AGA GAT GG-3^′
β-actin-r	969 – 950		5^′-AGC ACT GTG TTG GCG TAC AG-3^′
E2F-1-f	796 – 815	58	5^′-AGC TGG ACC ACC TGA TGA AT-3^′
E2F-1-r	945 – 926		5^′-GAG GGG CTT TGA TCA CCA TA-3^′
PCNA-f	444 – 463	56	5^′-GGC GTG AAC CTC ACC AGT AT-3^′
PCNA-r	688 – 669		5^′-TCT CGG CAT ATA CGT GCA AA-3^′