Potential Role of Thymosin-1 Adjuvant Therapy for Glioblastoma
Figure 5
Talpha1 enhances Granzyme B-mediated killing of 9L glioblastoma cells. 9L cells were pretreated with vehicle or Talpha1 for 3 hours. The cells were then divided and further treated with vehicle, Streptolysin O (permeabilizing agent to substitute for Perforin), Granzyme B only, or Granzyme B+Streptolysin O. After 0.1, 1, or 3 hours of incubation, the cells were harvested to determine viability (resistance to killing). Each experiment was performed in replicates of 4, and cell viability, measured with the ATP luminescence, was assayed in replicates of 6 or 8 and relative to control.
