Synergistic Induction of Apoptosis in Primary B-CLL Cells after Treatment with Recombinant Tumor Necrosis Factor-Related Apoptosis-Inducing Ligand and Histone Deacetylase Inhibitors
HDACi treatment sensitizes B-CLL cells to TRAIL-induced apoptosis. (a) B-CLL cells were cultured with or without 2.5 M Oxam or 500 ng/ml TSA or vehicle alone for 16 hours before addition of 100 ng/ml rhTRAIL. Cultures were harvested 4 hours later, and the percentage of cells undergoing apoptosis was determined by Annexin V staining. One experiment, representative of more than 5 experiments using cells from different donors, is shown. (b) The mean percentage of Annexin cells is shown for each treatment group. Cells were cultured as in (a).