Response of Patient-Derived Non-Small Cell Lung Cancer Xenografts to Classical and Targeted Therapies Is Not Related to Multidrug Resistance Markers

Table 1

Chemosensitivity testing, constitutive protein, and mRNA expression of BCRP, LRP, MDR1, and MRP1 in 26 xenografts and the positive controls. Response: − negativ: 100–50% T/C, + 35–50% T/C, ++ 21–35% T/C, +++ 6–20%T/C, ++++ 0–5% T/C, tox-toxic, n.t.—not tested; protein expression: − not detected, + weak, ++ medium, +++ strong expression; % of positive cells; mRNA expression: normalised ΔC_T values; etp—etoposide, carpl—carboplatin, gem—gemcitabine, paltx—paclitaxel, erlo—erlotinib.

						Before treatment
	Chemosensitivity					Protein expression				mRNA expression
LuCa	etp	Carpl	gem	paltx	erlo	BCRP	LRP	MDR1 [%]	MRP1 [%]	bcrp	lrp	mdr1	mrp1
7064	++	−	−	++	++	−	+	15.4	13.8	12.19	8.01	17.54	7.10
7126	−	−	+++	−	++	+++	+	15.3	18.2	4.17	5.04	18.18	3.45
7166	++	++	+	−	−	−	+++	13.7	13.8	15.46	6.45	0	7.16
7177	−	++	+++	−	++	−	++	14.5	29.5	10.42	5.33	16.97	2.02
7187	−	++	+++	−	−	−	++	15.4	18.3	8.13	6.90	0	7.55
7198	−	+	+	+	−	+++	++	27.2	20.9	10.13	6.18	21.24	6.25
7298	+	+	++	++	−	−	++	14.4	13.1	15.03	5.79	17.19	6.33
7336	−	(+)	++	+++	−	+++	+	13.3	13.6	8.08	6.68	0	7.02
7343	−	+++	+++	++	−	+	+	20.8	24.1	8.34	5.85	0	5.23
7387	−	−	+++	++++	−	++	++	15.4	29.9	6.15	7.64	10.90	7.57
7406	+	+	+++	+++	−	+++	+	29.4	22.4	14.87	7.61	14.97	8.10
7414	−	++	+++	+++	+	−	++	17.9	15.6	7.13	5.69	0	6.21
7433	−	+++	−	++++	−	−	+++	18.8	22.1	6.99	5.68	17.20	3.73
7462	−	+	++++	+++	++	+	−	39.8	31.3	7.65	5.24	9.36	7.78
7466	−	−	++++	++++	++	+	++	15.5	18.6	8.62	7.56	22.43	8.65
7506	−	++++	(+)	+++	−	+	+	65.8	19.0	10.96	7.07	15.94	6.98
7530	++++	−	tox	+++	−	+++	+	20.1	16.1	14.27	8.24	14.35	8.01
7558	−	++++	+++	−	−	+++	++	24.0	25.3	15.90	6.26	18.64	6.38
7612	−	++++	−	+++	−	−	+	17.7	15.5	11.09	5.89	16.98	6.04
7668	−	+++	tox	++++	−	+++	−	17.2	24.8	7.55	6.12	13.42	6.17
7700	−	−	++++	++	−	++	+++	18.1	13.9	15.81	6.01	17.32	7.96
7747	−	++	−	++	−	+	++	62.0	15.0	15.19	4.82	18.29	6.26
7766	−	+++	+	++	−	+	+	33.1	18.0	6.72	5.18	17.88	4.14
7860	+	−	++	+++	−	+	++	20.6	22.4	11.16	4.96	16.41	5.47
7913	−	+	(+)	+++	−	+	++	14.0	n.t.	13.50	5.64	18.47	6.17
7915	n.t	n.t	n.t	n.t	−	+	++	16.0	13.8	14.27	8.49	16.21	6.87
MDA-MB-231/BCRP										2.51
A549											9.97		5.54
MT3/ADR												3.49