Concordance of KRAS/BRAF Mutation Status in Metastatic Colorectal Cancer before and after Anti-EGFR Therapy

Table 1

Primer data for sequencing and allele specific PCR for KRAS exon 2 and BRAF exon 15 mutation analysis.

KRAS
𝑑1	5^′-GAG TTT GTA TTA AAA GGT ACT GG-3^′
𝑑2	5^′-TAC TGG TGG AGT ATT TGA TAG TG-3^′
𝑟1+𝑟2	5^′-CTG TAT CAA AGA ATG GTC CTG-3^′
BRAF
𝑑	5^′-TGC TTG CTC TGA TAG GAA AAT-3^′
𝑟	5^′-CTG ATG GGA CCC ACT CCA T-3^′