Lysophosphatidic Acid Upregulates Laminin-332 Expression during A431 Cell Colony Dispersal
RNA preparation. A431 cells were incubated for 24 h in serum-deprived culture medium. LPA (2 M) or PBS (control) was added to medium and incubated for 12 h at C in a humidified, 5% CO2, 95% air atmosphere. Total RNA was isolated from A431 cells treated with PBS or LPA (2 M) for 12 hrs, using a Qiagen RNeasy kit. The RNA integrity was measured by the RNA integrity number (RIN) software algorithm designed to classify the eukaryotic total RNA, based on numbering from 1 to 10 (1 indicates the most degraded RNA profile, and 10 indicates the most intact RNA). Electrophoresis of RNA in eukaryote total RNA Nano_DE114000902 resulted in an RNA integrity number (RIN) of 10.
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