Lysophosphatidic Acid Upregulates Laminin-332 Expression during A431 Cell Colony Dispersal

<table><i>cDNA microarray analysis. </i>Microarray analysis was performed on PBS and LPA-treated samples using Affymetrix Human U133 plus 2.0 chips according to manufacturer’s instructions, and data were normalized using the Microarray Suite 5.0 algorithm.  Among the upregulated genes induced by LPA, all three subchains of the intact Ln-332 molecule: <svg height="7.1750002" id="M72" style="vertical-align:-0.1254pt" version="1.1" viewbox="0 0 8.9375 7.1750002" width="8.9375" xmlns="http://www.w3.org/2000/svg">
<g transform="matrix(1.25,0,0,-1.25,0,7.175)">
<g transform="translate(72,-66.26)">
<text transform="matrix(1,0,0,-1,-71.95,66.44)">
<tspan style="font-size: 12.50px; " x="0" y="0">𝛼</tspan>
</text>
</g>
</g>
</svg>3 chain (LAMA3), laminin <svg height="13.425" id="M73" style="vertical-align:-2.29482pt" version="1.1" viewbox="0 0 8.8500004 13.425" width="8.8500004" xmlns="http://www.w3.org/2000/svg">
<g transform="matrix(1.25,0,0,-1.25,0,13.425)">
<g transform="translate(72,-61.26)">
<text transform="matrix(1,0,0,-1,-71.95,63.6)">
<tspan style="font-size: 12.50px; " x="0" y="0">𝛽</tspan>
</text>
</g>
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</svg>3 (LAMB3), and laminin <svg height="9.9375" id="M74" style="vertical-align:-2.34499pt" version="1.1" viewbox="0 0 8.0625 9.9375" width="8.0625" xmlns="http://www.w3.org/2000/svg">
<g transform="matrix(1.25,0,0,-1.25,0,9.9375)">
<g transform="translate(72,-64.05)">
<text transform="matrix(1,0,0,-1,-71.95,66.44)">
<tspan style="font-size: 12.50px; " x="0" y="0">𝛾</tspan>
</text>
</g>
</g>
</svg>2 chain (LAMC2) were significantly increased.</table>

Journal of Oncology

fig2

Figure 2

Figure 2: Lysophosphatidic Acid Upregulates Laminin-332 Expression during A431 Cell Colony Dispersal 