Lysophosphatidic Acid Upregulates Laminin-332 Expression during A431 Cell Colony Dispersal
Anti-Ln-332 antibody prevents A431 colony from dispersing by LPA treatment. Cell colony dispersal assays were performed similarly to our previous studies (serum-starved A431 cells for 24 h, treated with 2 M LPA), with the addition of an anti-Ln-332 antibody (BM165). (a, b) We found that anti-Ln-332 antibody significantly blocked dispersal of LPA-treated colonies, compared to those colonies treated with LPA alone (N = 3; P < .05). LPA-treated A431 colony dispersal percentages in the absence (LPA) or presence of BM165 antibody (LPA + anti-Ln-332) are indicated as light-grey triangles or cross, respectively. These results strongly support a general role for Ln-332 in regulating the “LPA effect”. P < .05, Two-way ANOVA (Bonferroni post test).
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