The Enigma of Tripeptidyl-Peptidase II: Dual Roles in Housekeeping and Stress

Table 2

Reported TPPII-dependency in processing of MHC class I-bound epitopes in live cells.

Source	MHC class I	Sequence	Cell type	In vivo^Ψ	Ref.^#Θ
Requirement for TPPII*
HIV-1 Nef73-82	HLA-A3/A11	QVPLRPMTYK^&@	DC, LCLs	—	[16]
EBV-LMP1	HLA-A2	YLLEMLRWL^&@	COS cells, LCLs	—	[19]
EBV-LMP1	HLA-A2	YLQQNWWTL^&@	COS cells, LCLs	—	[19]
Influenza NP147-155	H-2K^d	(TYQRTRALV)^&@#	HEK-293, L cells	—	[20, 49]^#
Listeria-LLO91-99	H-2K^d	GYKDGNEYI^&Δ	J774, BMM	—	[21]
Listeria-p60449-457	H-2K^d	IYVGNGQMI^&	J774, BMM	—	[21]
RU134-42	HLA-B51	VPYGSFKHV^&	BB-64 Renal carcinoma	—	[50]Θ
No requirement for TPPII
Listeria-p60217-225	H-2K^d	KYGVSVQDI^&	BMM	—	[21]
Listeria-p60476-484	H-2K^d	KYLVGFGRV^&	BMM	—	[21]
OVA	H-2K^b	SIINFEKL^&§	BMC-2, TPPII−/− DC	TPPII−/− GT^⋆	[18, 26, 27, 51]
LCMV NP118-126	H-2L^d	RPQASGVYM^&	J774	—	[26]
LCMV GP33-41	H-2K^b/D^b	KAVYNFATC^&§	BMC-2, HEK-293, TPPII−/− DC	TPPII−/−, GT	[18, 26, 27]
LCMV GP276-286	H-2D^b	SGVENPGGYCL^&§	BMC-2, HEK-293, TPPII−/− DC	TPPII−/−, GT	[18, 26, 27]
LCMV NP396-404	H-2D^b	FQPQNGQFI^&§	BMC-2, HEK-293, TPPII−/− DC	TPPII−/−, GT	[18, 26, 27]
LCMV NP205-212	H-2K^b	YTVKYPNL^&§	BMC-2	GT	[26, 27]
LCMV GP92-101	H-2D^b	CSANNSHHYI^&	BMC-2, HEK-293	—	[26]
MCMV- pp89168-176	H-2L^d	YPHFMPTNL^&	L cells	—	[26]
Epitope destruction by TPPII
Survivin	HLA-A2	ELTLGEFLKL^&	Colon carcinoma	—	[28]Θ

^∗The effect considered has to be at least partial in the processing of an epitope in live cells.
^Ψ𝐼n vivo dependence on TPPII for generation of a CTL response against epitope, the column contains the animal used.
^#The requirement for TPPII in the processing of this epitope is debated.
^ΘEvidence in some reports is based only on AAF-CMK, an inhibitor that is however not TPPII-specific.
Evidence base: catalytic inhibitor—&; siRNA—@; gene-deficient cells—x.
^⋆GT: Gene-trapped TPPII-deficient mouse.
^ΔOnly in the presence of proteasomal inhibitor.
Cells: Dendritic cells, COS: Chinese Hamster Ovary cells, LCL: Lymphoblastoid cells, HEK293: Human embryonic kidney cells, BMC-2 and J-774 - macrophage cell lines, BMM: Bone-marrow macrophages.