Thrombopoietin Receptor Levels in Tumor Cell Lines and Primary Tumors

Figure 3

Quantitative reverse transcription-polymerase chain reaction measurement of MPL, EPOR, ERBB2, and IGF1R mRNA in various normal tissue samples and tumor samples. Data were normalized to a set of housekeeping genes (GAPDH, PPIA, and ACTB). Cycle threshold (Ct) values were calculated using the following equation: Abundance=10e((40−Ct)/3.35). Samples were scaled relative to each other using the geometric mean of the set of valid housekeeping gene datapoints for that sample.

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