Low Level of Her-2 Locus Amplification by Fluorescent In Situ Hybridization Does Not Correlate with Her-2 Protein Overexpression by Immunohistochemistry in Barrett's Esophagus
Figure 1
Her-2 protein and Her-2 locus status as determined by IHC on BE biopsy and FISH on BE brush cytology specimens. (a) Strong overexpression of Her-2 protein (+3) in HGD, (b) moderate overexpression of Her-2 protein (+2) in EAC, (c) no overexpression of Her-2 protein (score 0) in EAC, (d) no overexpression of Her-2 protein (score 0) in LGD, (e) no overexpression of Her-2 protein (score 0) in ND, (f) high level of Her-2 locus amplification (clusters) in a cytology sample of a HGD case—CEP 17 (green) and Her-2 locus (red), (g) gain of chromosome 17 in a cytology sample of an EAC case—CEP 17 (green) and Her-2 locus (red), (h) low level of Her-2 amplification (ratio of Her-2: Cep17) in a cytology sample of an EAC case, CEP 17 (green) and Her-2 locus (red), (i) gain of chromosome 17 in a cytology sample of a LGD case—CEP 17 (green) and Her-2 locus (red), and (j) two copies of chromosome 17 (disomy) and Her-2 locus (normal pattern) in a cytology sample of a ND case—CEP 17 (green) and Her-2 locus (red).
