Low Level of Her-2 Locus Amplification by Fluorescent In Situ Hybridization Does Not Correlate with Her-2 Protein Overexpression by Immunohistochemistry in Barrett's Esophagus
Table 1
Her-2 protein overexpression and Her-2 locus/chromosome 17 status in BE and EAC cases.
No.
Histology
IHC
Brush cytology FISH
Tissue FISH
Her-2 expression
Her-2 locus amplification
CEP17
Her-2 locus amplification
CEP17
91
ND
0
—
Gain
Nd
Nd
237
ND
0
—
Gain
Nd
Nd
75
ND
0
—
Gain
Nd
Nd
148
ND
0
—
Gain
Nd
Nd
59
IND
1
—
Gain
Nd
Nd
134
IND
0
—
Gain
Nd
Nd
273
IND
1
—
Gain
Nd
Nd
98
LGD
0
—
Gain
Nd
Nd
255
LGD
0
—
Gain
Nd
Nd
3
HGD
0
—
Gain
Nd
Nd
4
HGD
3
High (50)
Gain (42)
High (39)
Gain (13)
5
HGD
0
Low (5)
Gain (71)
Low (10)
Gain (59)
152
HGD
0
—
Gain
Nd
Nd
167
HGD
1
High (8)
Gain (13)
Nd
Nd
170
HGD
2
Low (11)
—
Nd
Nd
173
HGD
3
High (50)
Gain (23)
Nd
Nd
193
HGD
0
Low (90)
Gain (6)
Low (55)
—
202
HGD
3
High (75)
Gain (83)
High (79)
Gain (84)
211
HGD
2
High (6)
Gain (11)
High (34)
Gain (34)
235
HGD
0
—
Gain
Nd
Nd
236
HGD
1
Low (20)
Gain (13)
Low (10)
Gain (53)
247
HGD
2
—
Gain
Nd
Nd
264
EAC
1
—
Gain
Nd
Nd
265
EAC
2
—
Gain
Nd
Nd
270
EAC
0
—
Gain
Nd
Nd
223
EAC
0
Low (8)
Gain
Nd
Nd
232
EAC
1
—
Gain
Nd
Nd
233
EAC
2
—
Gain
Nd
Nd
251
EAC
1
—
Gain
Nd
Nd
254
EAC
0
—
Gain
Nd
Nd
200
EAC
3
High (82)
—
Nd
Nd
274
EAC
0
—
Gain
Nd
Nd
276
EAC
3
High (50)
Gain (63)
High (42)
Gain (42)
250
EAC
0
—
Gain
Nd
Nd
FISH—fluorescent in situ hybridization; (—) indicates absence of certain abnormality; (gain)—ratio 1 : 1 of Her-2: Cep17, the number of signals in different cases varied from 3 to 6 signals per cell; (low)—low level amplification—ratio of Her-2: Cep17; (high)—high level amplification—ratio 5 : 1 of Her-2: Cep17; in the brackets % of abnormal cells IHC—immunohistochemistry; IHC score 0—no staining, IHC score +1—faint staining, IHC score +2—moderate staining, IHC score +3—strong staining; ND—no dysplasia; IND—indefinite for dysplasia; LGD—low grade dysplasia; HGD—high grade dysplasia.
