Review Article
Circulating Tumor Cell Analysis: Technical and Statistical Considerations for Application to the Clinic
Table 1
Determination of database/sample size that will provide a given precision in rare event analysis.
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For cell-based assays such as flow cytometry, a simple calculation can be used to determine the size of the database/sample that will provide a given precision: ; where is the number of events meeting the required criterion, and CV is the coefficient of variation of a known positive control. Modified from http://www.icms.qmul.ac.uk/flowcytometry/uses/rareeventanalysis/index.html, Queen Mary, University of London. With a WBC count in the low-normal range (/L), 10 mL of blood would contain events. Estimated frequency of CTCs in the peripheral blood of cancer patients. |