An Active Form of Sphingosine Kinase-1 Is Released in the Extracellular Medium as Component of Membrane Vesicles Shed by Two Human Tumor Cell Lines
Figure 5
Interactions of shed vesicles with endothelial cells. Vesicles shed by SK-Hep1 cells, labelled with lipid styryl dye FM4-64 (red fluorescence), were added to GM7373 endothelial cells in which β1 Integrin was stained using FITC-conjugated secondary antibodies (green fluorescence). Cells were incubated with vesicles, respectively, for (a) 10 minutes, (b) 20 minutes, and (c) 30 minutes. The arrows indicate vesicle localization.
