Targeting the Epidermal Growth Factor Receptor in Epithelial Ovarian Cancer: Current Knowledge and Future Challenges
Table 1
Summary of assays used in detecting EGFR in vitro and in vivo. Aside from high-throughput methods (such as cDNA arrays, comparative genomic hybridization, and reverse phase protein arrays) and xenograft tumor assays, more broadly encompassing biological methods such as assays for invasion, migration, or gene knockouts have been excluded. cDNA: complementary DNA; PCR: polymerase chain reaction.
Mass Spectrometry after Protein Enrichment /Purification (e.g., Immunoprecipitation, Chromatographic Separation, Baculovirus Expression)
Detection of protein modification sites (e.g., phosphorylation, glycosylation); changes in protein levels or proteomic profiles, protein-protein complexes
No
Microscopic Techniques (e.g., Confocal)
Determination of presence, location, or amount of protein in cell
No
Mulitplex Antibody Arrays (Solid Phase or Bead Based)
Detection of multiple molecules (usually proteins) of interest