Thrombospondin-1 (TSP-1) Stimulates Expression of Integrin 6 in Human Breast Carcinoma Cells: A Downstream Modulator of TSP-1-Induced Cellular Adhesion
Figure 3
TSP-1 induces integrin 6 protein expression in human breast cancer cells. MD-MBA-231 cells (a) and MD-MBA-435 cells (b) were grown in six well tissue culture plates in serum-free media either with or without 60 g/mL TSP-1 for 24 hours. Cell extracts were prepared with SDS-sample buffer, reduced with -mercaptoethanol, and separated on 10% SDS-PAGE. Blots were probed with 1 g/mL of mouse 6 integrin IgG and followed by 0.1 g/mL HRP-coupled rabbit antimouse IgG and developed using enhanced chemoluminesence. Experiments were repeated two times and the results of a representative experiment are shown in the figure.
