Significant Growth Inhibition of Canine Mammary Carcinoma Xenografts following Treatment with Oncolytic Vaccinia Virus GLV-1h68

Table 1

Viral titer in tissue samples (pfu/organ or tumor).

Animal no./dpi	1/21 dpi	2/21 dpi	3/21 dpi	4/42 dpi	5/42 dpi
Liver	120	1716	840	n.d	n.d
Lungs	1520	480	n.d.	56	324
Kidneys	n.d.	n.d.	n.d.	NT	NT
Spleen	150	n.d	n.d	n.d	n.d
Ovaries	n.d.	n.d	30	NT	NT
Tumor	$2\times {10}^7$	$1\times {10}^7$	$2.1\times {10}^7$	$7.3\times {10}^5$	$7.3\times {10}^5$

Tumor-bearing mice were injected with $5\times {10}^6$ pfu of GLV-1h68. Mice were sacrificed at day 21 or 42 after virus injection (dpi). The data were determined by standard plaque assays on CV-1 cells using aliquots of the homogenized organs and were displayed as mean pfu/organ or tissue. For each organ, two aliquots of 0.1 ml were measured in triplicates.
n.d.: not detected (detection LIMIT$<$10 pfu/organ).
NT: not tested.