Cancer Malignancy Is Enhanced by Glyceraldehyde-Derived Advanced Glycation End-Products

Figure 4

Cells were pre-incubated with or without 1 mM NAC for 30 min and then additionally incubated with control unglycated BSA or Glycer-AGEs for 1 h (a) or 20 h (b). (a) The active forms of Rac1 were precipitated with GST-Pak1-PBD and immobilized glutathione discs and analyzed by Western blotting using monoclonal anti-Rac1 antibody. At the same time, the total Rac1 in the sample lysates was detected, and the intensity of the band was quantified. (b) Migration assay. Data are shown as the mean $\pm \mathrm{\hspace{0.17em}\hspace{0.17em}}$SD ($n\mathrm{\hspace{0.17em}\hspace{0.17em}}=\mathrm{\hspace{0.17em}\hspace{0.17em}}3$) ${}^{**}P\mathrm{\hspace{0.17em}\hspace{0.17em}}<\mathrm{\hspace{0.17em}\hspace{0.17em}}.01$ versus control unglycated BSA, ${}^{\#\#}P\mathrm{\hspace{0.17em}\hspace{0.17em}}<\mathrm{\hspace{0.17em}\hspace{0.17em}}.01$ versus Glycer-AGEs.

(a)

(b)