Downregulation of Epidermal Growth Factor Receptor Expression Contributes to -TEA's Proapoptotic Effects in Human Ovarian Cancer Cell Lines
Figure 1
Effects of -TEA on cell growth and apoptosis in A2780S and A2780/CP70R cells. (a) A2780S and A2780/CP70R cells were plated, cultured and treated as described in Section 2. Assessment of viable cell numbers (absorbance) was determined by the MTS assay following treatment with different doses of -TEA for 1, 2 and 3 days. (b) Following treatment of cells with various concentrations of -TEA or vehicle control for 1, 2, or 3 days, floating and adherent cells were harvested, washed, and stained with DAPI. Apoptosis was determined by counting the number of cells exhibiting condensed and fragmented nuclear morphology, and reported as percent apoptosis. Data in (a) and (b) are depicted as the mean ± SD for three independent experiments ( in comparison to vehicle control).
