Downregulation of Epidermal Growth Factor Receptor Expression Contributes to 𝛼-TEA's Proapoptotic Effects in Human Ovarian Cancer Cell Lines

Figure 2

Two antiapoptotic molecules, FLIP and survivin, are downregulated by $\alpha$-TEA in both cell types, and overexpression of either FLIP or survivin blocks $\alpha$-TEA-induced apoptosis. (a) Cells were treated with α-TEA for various time periods and whole cell lysates were analyzed by western immunoblotting. ((b) and (c)) Cells were transiently transfected with empty vector control (pcDNA3), survivin (myc-survivin)- or FLIP (his-FLIP-L)-expression plasmids. After 24 hours of transfection, cells were treated with or without $\alpha$-TEA (20 or 40 $\mu$M for A2780S and A2780/CP70R, resp.) for 1 day prior to DAPI analysis for apoptosis (b), and for 12 hours prior to immunoblot analysis (c). Data in (a) and (c) are representative of a minimum of two independent experiments. Data in (b) are depicted as the mean $\pm$ SD for three independent experiments (${}^{*}=P<.001$ in comparison to vector control).

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