Downregulation of Epidermal Growth Factor Receptor Expression Contributes to 𝛼-TEA's Proapoptotic Effects in Human Ovarian Cancer Cell Lines

Figure 4

$\alpha$-TEA downregulates ErbB1, -2, or -3 receptors in A2780S and A2780/CP70R cells. A2780S and A2780/CP70R cells were treated with 20 $\mu$M and 40 $\mu$M of $\alpha$-TEA, respectively, for 3, 6, 9, and 12 hours or vehicle control for 12 hours. Cell lysates from treatment groups were analyzed by immunoblotting for ErbB1, ErbB2, ErbB3 and ErbB4 protein levels. GAPDH levels were determined to verify lane loads for densitometric analyses with vehicle control given a value of 100. Data are representative of two or more experiments.