Association of ABCC2 and CDDP-Resistance in Two Sublines Resistant to CDDP Derived from a Human Nasopharyngeal Carcinoma Cell Line

Figure 3

Intracellular accumulation of CDDP. (a) A typical chromatogram for total analysis of CDDP using HPLC determination. (b) Calibration curve for gradient concentration of CDDP within the range of 5–80 $\mu$g/ml. A typical linear relationship ($R^2$ = 0.9965) was found between peak height and concentration of cisplatin. (c) The cellular accumulation of CDDP in parental CNE2 and resistant sublines. The concentration of CDDP was determined according to the calibration curve of CDDP. CNE2 resistant sublines were treated with or without PSC 833. ${}^{*}$ P  <  .05 versus CNE2.

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