Hyperphosphorylated FAK Delocalizes from Focal Adhesions to Membrane Ruffles

Figure 2

Increased phosphorylated FAK in membrane ruffles upon PV treatment. (a) Cells expressing FAK/Ycam were treated with vehicle or PV for 20 minutes, PFA fixed and immunostained with phospho-specific FAK (Tyr397 and Tyr861) Abs. Note the increase in global fluorescence in PV treated cells. Arrowheads indicate the presence of phospho-FAK in membrane ruffles. Scale bar, 10 $\mu$m. (b) Graphs present p-Y397-FAK and p-Y861-FAK intensities relative to total FAK intensity (YFP). Data are means $\pm$ s.e.m. from 6 independent experiments.

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