CXCL7-Mediated Stimulation of Lymphangiogenic Factors VEGF-C, VEGF-D in Human Breast Cancer Cells

Figure 1

CXCR2 antagonist SB225005 reduced the invasion of MCF10AT stably transfected with CXCL7 using a BD BioCoat Matrigel invasion assay (6-well plates), 1.25 x 10⁵ cells/ml were inoculated onto the membrane in serum-free medium. For the blocking experiment, there was serum-free medium containing SB225002 (1.1 $\mu$M) in the upper chamber. The lower chamber contained complete medium, and the control membrane did not have Matrigel by which to measure the migration of cells. After 48 hours, membranes were fixed, stained, and photographed, then the percent invasion was determined. Triplicate assays were done. Results are reported per cell line as percent invasion $\pm$ (mean number of cells invading Matrigel membrane/mean number of cells migrating through control membrane) $\times 100$ ($P<.01$). (Upper) SB225002 significantly decreased the invasion of CXCL7-stable transfected MCF10AT cells compared with notreated cells. (Lower) Membranes were stained by Diff-Quik kit and were photographed (original magnification x 20).