Evaluation of Lentiviral-Mediated Expression of Sodium Iodide Symporter in Anaplastic Thyroid Cancer and the Efficacy of In Vivo Imaging and Therapy

Figure 3

Detection of integrated hNIS sequence and RNA expression Lentiviral integration of hNIS construct was confirmed by PCR amplifying the specific DNA fragment in genomic DNA. Lane 4 and 5 represent the result of amplifying integrated hNIS fragment from isolated genomic DNA of parental and transduced cells, respectively. As assayed by RT-PCR, lane 1 and 2 represent the RNA expression of exogenous hNIS of parental and transduced cells, respectively. Same reaction was also carried out for detecting RNA expression of β-actin and was shown in all lanes. Lane 3 represents the PCR result with hNIS-containing plasmid as the template which was a control.