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Journal of Oncology
Volume 2011, Article ID 232037, 11 pages
Research Article

Antimyeloma Effects of the Heat Shock Protein 70 Molecular Chaperone Inhibitor MAL3-101

1Division of Hematology/Oncology, Department of Medicine, SUNY Downstate Medical Center, Brooklyn, NY 11203, USA
2Department of Biological Sciences, University of Pittsburgh, Pittsburgh, PA 15260, USA
3Department of Biology, Clarion University, Clarion, PA 16214, USA
4Department of Biochemistry and Biophysics, Howard Hughes Medical Institute, University of California, San Francisco, CA 94158, USA
5Department of Chemistry and Center for Chemical Methodologies and Library Development, University of Pittsburgh, Pittsburgh, PA 15260, USA

Received 21 May 2011; Accepted 18 July 2011

Academic Editor: Edward A. Copelan

Copyright © 2011 Marc J. Braunstein et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

Comparison of the inhibitory concentrations (IC50) of indicated combinations of MAL3-101, MG-132 and 17-AAG in NCI-H929 cells (compared to DMSO-treated control cells) are shown in Table S1. Structures of MAL3-101 and MAL3-51 are shown in Figure S1. Dot plots shown in Figure S2 illustrate apoptosis in NCI-H929 cells caused by exposure to indicated concentrations of MAL3-101, MG-132, or their combination obtained by dual Annexin V and propidium iodide (PI) staining and flow cytometry.

Figure S3 shows preliminary in vivo tumor progression experiments where NSG mice were treated i.p. 20 mg/kg MAL3-101 either 24 h after tumor inoculation (n=1) or 8 d after tumor inoculation (n=1) as indicated. Each drug schedule was controlled with an animal treated with vehicle. All mice (n=4) were inoculated subcutaneously in the right flank with 3 × 107 NCI-H929 cells. Treatment with 20 mg/kg MAL3-101 or vehicle was given twice weekly via i.p. The day of tumor inoculation is considered Day 0 on the x-axis. Tumor volume analyses for days 0, 14 and 29 after tumor inoculation are shown. Mean tumor volumes are shown for vehicle-treated animals.

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