Detection of EpCAM-Negative and Cytokeratin-Negative Circulating Tumor Cells in Peripheral Blood
Figure 1
Diagram of the CEE microchannel. (a) Top view of the channel showing the inlet where sample is loaded and the outlet that is attached to a syringe pump to draw sample through the channel. (b) Bottom view shows the area where 9,000 posts are located in the silicone block and the channel sealed with the bottom cover slip. The total volume of the microchannel is 24 μL. A standard microscope slide is added for stability during handling but is removed to visualize cells. The microchannel is inverted on a microscope and the captured cells viewed through the coverslip.
