Detection of EpCAM-Negative and Cytokeratin-Negative Circulating Tumor Cells in Peripheral Blood
Figure 3
Capture and stain of SKOV cells with EpCAM and with an antibody mixture. (a) The relative stain intensity of these cells on a microscope slide using anti-mouse-AlexaFluor488 when the cells were preincubated with anti-EpCAM antibody only, or with an antibody mixture of anti-HER2/neu, anti-CD44 and anti-CD28. (b) The FACS profile of the respective antigens of EpCAM or of the 3-antibody mixture present on each cell. (c) The percentage capture of SKOV cells when preincubated with anti-EpCAM or the antibody mixture. This shows that much lower antigen levels are necessary for good cell capture than for good staining intensity. Antibody mixtures improve CE staining efficiency.
