Research Article
Antigenic and Genotypic Similarity between Primary Glioblastomas and Their Derived Neurospheres
Table 1
List of antibodies.
| | Antibody | Source | Dilution IHC | Dilution WB | Code | Company |
| | Nestin*,° | Mouse | 1 : 200 | — | MAB5326 | Chemicon | | Nestin*,° | Rabbit | 1 : 200 | 1 : 1000 | AB5922 | Chemicon | | GFAP° | Mouse | 1 : 200 | — | M0761 | DakoCytomation | | GFAP° | Rabbit | 1 : 200 | 1 : 1000 | Z0334 | DakoCytomation | | CD133° | Mouse | 1 : 20 | 1 : 250 | 130-090-422 | Miltenyi Biotec | | Musashi-1° | Rabbit | 1 : 200 | 1 : 500 | AB5977 | Chemicon | | SOX2*,° | Rabbit | 1 : 50 | — | sc-20088 | Santa Cruz Biotec. | | SOX2*,° | Mouse | 1 : 100 | 1 : 250 | MAB2018 | R&D Systems | | REST*,° | Rabbit | 1 : 150 | — | IHC-00141 | Bethyl Laboratories | | REST | Rabbit | — | 1 : 2500 | A300-540A | Bethyl Laboratories | | GalC*,° | Mouse | 1 : 200 | — | MAB342 | Chemicon | | βIII-Tubulin*,° | Mouse | 1 : 250 | — | MAB1637 | Chemicon | | Ki-67/MIB-1* | Mouse | 1 : 100 | — | M7240 | DakoCytomation | | MGMT | Mouse | — | 1 : 400 | MS-470-P0 | Lab Vision Corp. | | α-Tubulin | Rabbit | — | 1 : 5000 | LF-PA0146 | LabFrontier | | CD34 | Mouse | Pre-diluted | — | 790-2927 | Ventana |
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*HIER required.
°tested by IF.
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