Antigenic and Genotypic Similarity between Primary Glioblastomas and Their Derived Neurospheres

Table 2

Microsatellites markers used for LOH analysis.

Region	STR	Locus	Repeat	Max. het*	PCR primers 5′ to 3 (forward/reverse)
1p	D1S508	1p36.23	(AC)n	0.812	AGCTGGGGAATATATGTNTCATATTGTGGAAGGCCAACTC
	D1S496	1p36.1-p34.3	(AC)n	0.815	TCTCTATGCATCTATGCATCTATCTGCATTTGTCTGGGTTCTT
	D1S2724	1p33	(AC)n	0.821	TCAAGTCCCAGGAGGATTCCACTGTGTTATTTAGCAGGAT
	D1S457	1p13.3	(AC)n	0.742	GGGGGCAATAACACAAAGGAGCCTGGAGCCAAGAGTGCTA
9p	D9S157	9p22.2	(AC)n	0.849	AGCAAGGCAAGCCACATTTCTGGGGATGCCCAGATAACTATATC
	D9S171	9p21.1	(AC)n	0.804	AGCTAAGTGAACCTCATCTCTGTCTACCCTAGCACTGATGGTATAGTCT
10q	D10S212	10q26.3	(AC)n	0.74	GAAGTAAAGCAAGTTCTATCCACGGAAGTAAAGCAAGTTCTATCCACG
	D10S190	10q26.11	(AC)n	0.863	GTGTTTGGGTCATGGAGATGAGGCAAAGCAGGAGCA
	D10S562	10q25.3	(AC)n	0.715	CCTGGCAGATGGAGGTTTCGGAGTGCTTCCTTAAAATAC
17p	TP53 Alu	17p13.1	(AAAAT)n	0.594	ACTCCAGCCTGGGCAATAAGAGCTACAAAACATCCCCTACCAAACAGC
	D17S520	17p13	(AC)n	0.854	GGAGAAAGTGATACAAGGGATAGTTAGATTAATACCCACC
19q	D19S412	19q13.33	(AC)n	0.812	TGAGCGACAGAATGAGACTACATCTTACTGAATGCTTGC
	D19S219	19q13.32	(AC)n	0.77	GTGAGCCAAGATTGTGCCGACTATTTCTGAGACAGATTCCCA

STR: Short Tandem Repeat.
*Maximum heterozygosity.