α2 Integrin-Dependent Suppression of Pancreatic Adenocarcinoma Cell Invasion Involves Ectodomain Regulation of Kallikrein-Related Peptidase-5
Figure 4
Stable α2 reexpression upregulates KLK-5, 6, and 7, which are expressed in a differentiation-dependent manner and regulate in vitro invasion and collagenI migration. (a) (i) Heatmap presentation of identified gene products. (ii) RT-PCR analysis of Affymetrix-identified candidates KLK-5, 6, and 7 in MP2-mock, MP2-α2/TC and MP2-α2/CI cells. GAPDH, control. (iii) RT-PCR analysis of KLK-5, 6 and 7 expression in a spectrum of pancreatic cells including: untransformed (HPDE), well-(CAPAN1 and CAPAN2), moderately (BxPC3 and COLO357) and poorly differentiated PDAC (Panc1 and PT45P1) (see Supplemental Table S1 for information on cellular grade and origin). GAPDH, control. (b) Invasion analysis of the indicated cells in the presence or absence of KLK inhibitor (KLKi). (c) CollagenI migration analysis of the indicated cells in the presence or absence of KLK inhibitor (KLKi).
