α2 Integrin-Dependent Suppression of Pancreatic Adenocarcinoma Cell Invasion Involves Ectodomain Regulation of Kallikrein-Related Peptidase-5
Figure 5
KLK5 mediates an anti-invasion phenotype in α2-expressing PDAC cells in vitro. (a) Immunoblot analysis of the indicated cells for expression of KLK5, KLK6, and α2 integrin. Actin, loading control. (b) ELISA of KLK5 and KLK6 secretion into culture media conditioned by the indicated cells. (c) ELISA analysis of KLK5 and KLK6 secretion by engineered BxPC3 cells stably transfected with KLK5 (Bx/KLK5) and KLK6 (Bx/KLK6) versus hygromycin-resistant mock transfectants (Bx/mock). (d) Invasion (left panel) and collagenI migration (right panel) analysis of the indicated stable BxPC3 populations in the presence or absence of KLK inhibitor (KLKi). (e) ELISA analysis of KLK5 secretion by engineered MP2 cells stably transfected with KLK5 (MP2/KLK5) versus hygromycin-resistant mock transfectants (MP2/mock). αKLK6, control. (f) Invasion analysis of the indicated stable MP2 cells in the presence or absence of KLK inhibitor (KLKi). (g) Immunoblot analysis of total α2 expression by the indicated stable BxPC3 populations. Actin, control. (h) Invasion (left panel) and collagenI migration (right panel) analysis of the stable MP2/KLK5 cells transiently transfected with α2 (α2-48 h) or empty vector (mock).
