Journal of Oncology / 2011 / Article / Fig 4

Research Article

Peroxisome Proliferator-Activated Receptor-γ Ligands Alter Breast Cancer Cell Motility through Modulation of the Plasminogen Activator System

Figure 4

Ciglitazone treatment increases uPA activity in MCF-10A cells (a) but not in MCF-10CA1 cells (b). Following 24-hour treatment with ciglitazone (0–5 μM), in the absence and presence of 5 μM GW9662, media was removed from cells, washed in 1x PBS and plasminogen was then added, after 30-minutes at room temperature, cell supernatant was transferred to wells containing plasmin chromogenic substrate (S-2251, Chromogenix). Kinetics were read at 405 nm for 1.5-hours at 37°C. Values represent average at Ab 405 nm, normalized to no treatment control, each condition done in triplicate .

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