Journal of Oncology / 2011 / Article / Fig 5

Research Article

Peroxisome Proliferator-Activated Receptor-γ Ligands Alter Breast Cancer Cell Motility through Modulation of the Plasminogen Activator System

Figure 5

Arachidonic acid treatment increases MCF-10A cell motility (a) and uPA activity (b). MCF-10A cells were treated for 24-hours in serum-free media containing 10 μM ArA or vehicle control (absolute ethanol in DMEM:F12 containing 1 mg/mL fatty acid-free BSA) following a 30 min pretreatment with GW9662 (5 μM) or vehicle (DMSO) as detailed under Section 2. Values represent average number of cells chemotaxing to EGF/well + SD. . Separately, after receiving the same treatment as above, cells were washed in PBS, then incubated in buffer containing plasminogen. Supernatant from cells was transferred to a new well containing buffer, amiloride and plasmin substrate. Values represent average (Abs 405 nm) + SD .

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