Gene Expression Differences between Enriched Normal and Chronic Myelogenous Leukemia Quiescent Stem/Progenitor Cells and Correlations with Biological Abnormalities
Figure 4
Comparing the total cloning efficiencies (C.E.s) of CD34+ G0 and G1/S/G2M cells from normal bone marrow, normal mobilized peripheral blood, pooled cord blood samples, and chronic phase CML peripheral blood samples. Total Cloning Efficiencies (CFU-GM+CFU-E/BFU-E) at 14-15 days of normal and CML CD34+ G0 and G1/S/G2/M cells after near-maximal stimulation with 5–7 cytokines, all without EPO: (a) CD34+ G0 and G1/S/G2/S cells from 3 normal bone marrow samples, 2 normal mobilized peripheral blood samples, 5 pooled Cord Blood samples, and 10 CML peripheral blood samples. The values for the cord blood CD133+ and CD133- bars shown are the average of 3 separate pooled cord blood experiments. (b) The same CD34+ G0 only samples showing proportions of myeloid (CFU-GM) and erythroid (CFU-E+BFU-E) colonies. (c) The same as B but CD34+ G1S/G2/M cells.
