798592.fig.006a
(a)
798592.fig.006b
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798592.fig.006c
(c)
Figure 6: (a) Cloning of CD34+ G0 and G1/S/G2/M cells enriched from pooled peripheral blood samples of 2 untreated chronic phase CML. The total C.E.s were low, and almost all of both the GM and erythroid colonies were tiny, small, or medium sized with very few large colonies and with very few or no colonies after stimulation with 3 growth factors (KL+FL+TPO), pointing out the variable quality of the CML samples received. In this case, both the G0 CD133 positive and negative fractions formed almost entirely small erythroid clusters and colonies with 7 GFs, but only the CD133 negative G1/S/G2/M cells produced colonies, including some GM colonies. (b) Colony formation by G0 and G1/S/G2/M cells enriched from the peripheral blood of a chronic phase CML patient. The sample produced GM colonies with fairly high C.E.s following stimulation with 3 or 7 cytokines without EPO. With additional enrichment of the G0 cells (G0 CD133/2+) the C.E.s further increased by about a third, with production of almost entirely GM colonies. (c) Maximum stimulation of G0 and G1/S/G2/M cells enriched from the peripheral blood of a chronic phase CML patient.The G0 cells had a high total C.E. after stimulation with 8 cytokines shown, including EPO. Even with addition of EPO, while both the G0 and G1/S/G2/M CD133 negative cells produced predominately large, X-large erythroid, and mixed colonies, the CD133 positive fractions produced mainly GM colonies, but also about 20% large and X-large BFU-E plus mixed colonies.