A Cohort Study of p53 Mutations and Protein Accumulation in Benign Breast Tissue and Subsequent Breast Cancer Risk

Table 2

p53 primer sequences and PCR conditions.

Primers	Sequences	Size	PCR conditions
Exon 5	5′-GCTGCCGTGTTCCAGTTGCT-3′	294 bp	95°C, 50 sec; 58°C, 50 sec; 72°C, 60 sec, 35 cycles
	5′-CCAGCCCTGTCGTCTCTCCA-3′
Exon 6	5′-GGCCTCTGATTCCTCACTGA-3′	199 bp	95°C, 50 sec; 55°C, 50 sec; 72°C, 60 sec, 35 cycles
	5′-GCCACTGACAACCACCCTTA-3′
Exon 7	5′-TGCCACAGGTCTCCCCAAGG-3′	196 bp	95°C, 50 sec; 56°C, 50 sec; 72°C, 60 sec, 35 cycles
	5′-AGTGTGCAGGGTGGCAAGTG-3′
Exon 8	5′-CCTTACTGCCTCTTGCTTCT-3′	225 bp	95°C, 50 sec; 55°C, 50 sec; 72°C, 60 sec, 35 cycles
	5′-ATAACTGCACCCTTGGTCTC-3′
Exon 9	5′-GCCTCAGATTCACTTTTATCACC-3′	152 bp	95°C, 50 sec; 56°C, 50 sec; 72°C, 60 sec, 35 cycles
	5′-CTTTCCACTTGATAAGAGGTCCC-3′
Exon 10	5′-TGATCCGTCATAAAGTCAAACAA-3′	236 bp	95°C, 50 sec; 56°C, 50 sec; 72°C, 60 sec, 35 cycles
	5′-GGAGTAGGGCCAGTAAGGG-3′