Journal of Oncology / 2011 / Article / Tab 2

Research Article

A Cohort Study of p53 Mutations and Protein Accumulation in Benign Breast Tissue and Subsequent Breast Cancer Risk

Table 2

p53 primer sequences and PCR conditions.

PrimersSequencesSizePCR conditions

Exon 55′-GCTGCCGTGTTCCAGTTGCT-3′294 bp95°C, 50 sec; 58°C, 50 sec; 72°C, 60 sec, 35 cycles
5′-CCAGCCCTGTCGTCTCTCCA-3′

Exon 65′-GGCCTCTGATTCCTCACTGA-3′199 bp95°C, 50 sec; 55°C, 50 sec; 72°C, 60 sec, 35 cycles
5′-GCCACTGACAACCACCCTTA-3′

Exon 75′-TGCCACAGGTCTCCCCAAGG-3′196 bp95°C, 50 sec; 56°C, 50 sec; 72°C, 60 sec, 35 cycles
5′-AGTGTGCAGGGTGGCAAGTG-3′

Exon 85′-CCTTACTGCCTCTTGCTTCT-3′225 bp95°C, 50 sec; 55°C, 50 sec; 72°C, 60 sec, 35 cycles
5′-ATAACTGCACCCTTGGTCTC-3′

Exon 95′-GCCTCAGATTCACTTTTATCACC-3′152 bp95°C, 50 sec; 56°C, 50 sec; 72°C, 60 sec, 35 cycles
5′-CTTTCCACTTGATAAGAGGTCCC-3′

Exon 105′-TGATCCGTCATAAAGTCAAACAA-3′236 bp95°C, 50 sec; 56°C, 50 sec; 72°C, 60 sec, 35 cycles
5′-GGAGTAGGGCCAGTAAGGG-3′

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