Toll-Like Receptor 7 Agonist Therapy with Imidazoquinoline Enhances Cancer Cell Death and Increases Lymphocytic Infiltration and Proinflammatory Cytokine Production in Established Tumors of a Renal Cell Carcinoma Mouse Model
Figure 4
3M011 increases cell death in mouse RCC tumors. (a), (b) Representative example of a positive TUNEL patch of tissue at 100x (a) and 200x (b) magnification in a tumor treated 2 days prior with 3M011 imidazoquinoline; patchy tissue TUNEL positivity was also observed in placebo-treated tumors (not pictured). (c), (d) Representative example of negative cleaved caspase-3 immunostain at 200x (c) and 400x (d) magnification in a placebo-treated tumor, including a tissue patch with corresponding TUNEL positivity (bottom two-thirds of each picture). (e), (f) Representative example of diffusely positive cleaved caspase-3 immunostain at 200x (e) and 400x (f) magnification in a large tissue region corresponding to TUNEL positivity from a 3M011-treated tumor. (g), (h) Mean percentage of tissue staining positive for TUNEL (g) and cleaved caspase-3 (h) among tumors treated 2 days prior with placebo versus 3M011. *; **; error bars = standard error of the mean.
