Bone Marrow Suppression by c-Kit Blockade Enhances Tumor Growth of Colorectal Metastases through the Action of Stromal Cell-Derived Factor-1
Figure 2
Semiquantitative analysis of the onset of angiogenesis within the CT 26.WT-GFP tumors. Animals were pretreated with anti-c-Kit (grey triangles) alone or anti-c-Kit and anti-SDF-1 neutralizing antibodies (black squares). Animals treated with isotype-matched control antibodies served as controls (white circles). In controls and anti-c-Kit pretreated animals, ~50% of the ROIs showed newly developed microvessels 5 days after tumor cell implantation. By day 8, almost 100% of the ROIs were vascularized. Treatment with anti-c-Kit and anti-SDF-1 resulted in a significant delay of angiogenesis with only ~50 and ~70% of the ROIs showing newly developed microvessels by days 8 and 11. Within these tumors, angiogenesis could be observed in all ROIs only at day 14. Mean ± SEM; * versus control; # versus anti-c-Kit.
