Bone Marrow Suppression by c-Kit Blockade Enhances Tumor Growth of Colorectal Metastases through the Action of Stromal Cell-Derived Factor-1
Figure 3
Time course of the functional capillary density within CT-26.WT-GFP tumors in dorsal skinfold chambers analyzed by intravital fluorescence microscopy. Representative fluorescence microscopic images show the network of chaotically arranged microvessels within the tumor center of control animals (a), animals pretreated with anti-c-Kit (b), and animals additionally treated with anti-SDF-1 (c) at day 11 after tumor cell implantation. Quantitative analysis of the functional capillary density (d) revealed a significant inhibition of tumor neovascularization within the tumor center after combined anti-c-Kit and anti-SDF-1 treatment (black squares) compared to controls (white circles). Anti-c-Kit pretreatment alone (grey triangles) did not significantly influence the extent of neovascularization. Mean ± SEM; * versus control; # versus anti-c-Kit. Original magnification (a)–(c) ×40.
